A01

Downstream signalling of hippocampal ensembles in the entorhinal cortex

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Local ensembles form complex spatiotemporal activity patterns. How is this information transferred from one network to others?

We address this question in the murine hippocampal-entorhinal system. Multi-neuronal ensembles in the hippocampus form transient representations of space and context. These take the form of highly organized spike patterns during different types of network oscillations. The “two-stage” model of memory formation suggests that such patterns are later transferred ...

How much information is preserved when activity propagates from CA1 to the EC?
Does postsynaptic activity of single EC neurons differ for input from distinct ensembles?
Do EC neurons preserve the very precise timing of hippocampal activity patterns?
Does repeated input from CA1 ensembles induce lasting plastic changes in the EC?
We tackle these questions in the hippocampal entorhinal-cortex slices, using a large variety of electrophysiological and optogenetic techniques as well as advanced data analysis. Parallel experiments are performed by in vivo-recordings of field potentials and single-cell activity. Together, these experiments shall shed light on the qualitative and quantitative properties of signal propagation between local networks.
Publications
Fischer V, Both M*, Draguhn A, Egorov AV (2014) Choline-Mediated Modulation of Hippocampal Sharp Wave-Ripple Complexes in vitro. J Neurochem doi: 10.1111/jnc.12693.

Zylla MM, Zhang X, Reichinnek S, Draguhn A, Both M* (2013) Cholinergic plasticity of oscillating neuronal assemblies in mouse hippocampal slices. PLoS One 8:e80718.

Traub RD, Schmitz D, Maier N, Whittington MA, Draguhn A (2012) Axonal properties determine somatic firing in a model of in vitro CA1 hippocampal sharp wave/ripples and persistent gamma oscillations. Eur J Neurosci 36:2650-2660.

Reichinnek S, von Kameke A, Hagenston AM, Freitag E, Roth FC, Bading H*, Hasan MT, Draguhn A, Both M* (2012) Reliable optical detection of coherent neuronal activity in fast oscillating networks in vitro. Neuroimage 60:139-152.

Bähner F, Weiss EK, Birke G, Maier N, Schmitz D, Rudolph U, Frotscher M, Traub RD, Both M*, Draguhn A (2011) Cellular correlate of assembly formation in oscillating hippocampal networks in vitro. Proc Natl Acad Sci U S A 108:E607-616.

Reichinnek S, Künsting T, Draguhn A, Both M* (2010) Field potential signature of distinct multicellular activity patterns in the mouse hippocampus. J Neurosci 30:15441-15449.

Both M*, Bahner F, von Bohlen und HO, Draguhn A (2008) Propagation of specific network patterns through the mouse hippocampus. Hippocampus 18:899-908.

Maier N, Nimmrich V, Draguhn A (2003) Cellular and network mechanisms underlying spontaneous sharp wave-ripple complexes in mouse hippocampal slices. JPhysiol 550:873-887.

* Principal investigators of other projects within the CRC