B01

Local and expanding glial activity patterns in memory-related networks of mice

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The most abundant cells in the brain are not neurons but glia. Do they play an active role in information processing?

The very specific focal or global activity of astroglia cells might be highly correlated with neuronal networks that are active in learning and memory. Our studies will reveal how focal [Ca2+]i-events are generated and propagated in astrogilal cell populations. Ultimately, we want to elucidate whether astrocytes show spatiotemporal population responses similar to, and in conjunction with, neuronal ensembles.

Recent findings using Ca2+-activity indicators provide first ideas how and why neuronal activity triggers specific activity patterns in astroglial networks in the hippocampus. However, it is still unsolved whether the controversially discussed neurotransmitter activation of astroglia is involved in learning and memory. To unravel putative correlations between specific patterns of neuronal and astroglial activity, we are planning to analyze an to compare intracellular [Ca2+]i-elevations in hippocampal astroglia populations during spontaneous, and during induced multicellular neuronal activity in adult, aged and learning impairment mice. As an ultimate goal we envisage doing in vivo recordings of astroglia activity in the olfactory bulb, somatosensory cortex and possibly in the hippocampus.
Publications
Bannerman DM, Bus T, Taylor A, Sanderson DJ, Schwarz I, Jensen V, Hvalby O, Rawlins JN, Seeburg PH, Sprengel R (2012) Dissecting spatial knowledge from spatial choice by hippocampal NMDA receptor deletion. Nat Neurosci 15:1153-1159.

Freudenberg F, Marx V, Mack V, Layer LE, Klugmann M, Seeburg PH, Sprengel R, Celekil T (2013). GluA1 and its PDZ-interaction: a role in experience-dependent behavioral plasticity in the forced swim test. Neurobiol Dis 52:160–167.

Hirrlinger J, Scheller A, Hirrlinger PG, Kellert B, Tang W, Wehr MC, Goebbels S, Reichenbach A, Sprengel R, Rossner MJ, Kirchhoff F (2009) Split-cre complementation indicates coincident activity of different genes in vivo. PLoS One 4:e4286.

Jensen V, Kaiser KM, Borchardt T, Adelmann G, Rozov A, Burnashev N, Brix C, Frotscher M, Andersen P, Hvalby O, Sakmann B, Seeburg PH, Sprengel R (2003) A juvenile form of postsynaptic hippocampal long-term potentiation in mice deficient for the AMPA receptor subunit GluR-A. J Physiol 553:843-856.

Lütcke H, Murayama M, Hahn T, Margolis DJ, Astori S, Zum Alten Borgloh SM, Gobel W, Yang Y, Tang W, Kugler S, Sprengel R, Nagai T, Miyawaki A, Larkum ME, Helmchen F, Hasan MT (2010) Optical recording of neuronal activity with a genetically-encoded calcium indicator in anesthetized and freely moving mice. Front Neural Circuits 4:9.

Mack V, Burnashev N, Kaiser KM, Rozov A, Jensen V, Hvalby O, Seeburg PH, Sakmann B, Sprengel R (2001) Conditional restoration of hippocampal synaptic potentiation in Glur- A-deficient mice. Science 292:2501-2504.

Saab AS, Neumeyer A, Jahn HM, Cupido A, Šimek AA, Boele HJ, Scheller A, Le Meur K, Götz M, Monyer H*, Sprengel R, Rubio ME, Deitmer JW, De Zeeuw CI, Kirchhoff F (2012) Bergmann glial AMPA receptors are required for fine motor coordination. Science 337: 749-753.

Tang W, Ehrlich I, Wolff SBE, Michalski A-M, Wölfl S, Hasan MT, Lüthi A, Sprengel R (2009) Faithful expression of multiple proteins via 2A-peptide self-processing: a versatile and reliable method for manipulating brain circuits. J Neurosci 29:8621-8629.

*Principal investigators of other projects within the CRC