Monitoring behaviourally relevant spatio-temporal activity patterns in prefrontal networks using in vivo wide-field microendoscopy
Decision making is a basic mental process for the control and execution of behaviour. Its impairment may result in impulsive and compulsive actions, both key features in many psychopathologies including addiction and eating disorders. Whether or not a response will be executed is critically determined by the medial prefrontal cortex (mPFC). This control function is accomplished by the interplay of mPFC subregions, in particular between the prelimbic (PL) and infralimbic (IL) areas as shown by classical lesion and stimulation methods. An unresolved question is how distinct neuronal populations within the mPFC contribute to specific task execution.
Tapocik J, Solomon M, Flanigan M, Meinhardt M, Barbier E, Schank J, Schwandt M, Sommer MH§, Heilig M§ (2013) Coordinated dysregulation of mRNAs and microRNAs in the rat medial prefrontal cortex following a history of alcohol dependence Pharmacogenomics J 13:286-296.§equal contribution
Körber C*, Horstmann H, Sätzler K, Kuner T (2012) Endocytic structures and synaptic vesicle recycling at a central synapse in awake rats. Traffic 13:1601-1611.
Hermann D, Weber-Fahr W, Sartorius A, Hoerst M, Frischknecht U, Tunc-Skarka N, Perreau-Lenz S, Hansson AC*, Krumm B, Kiefer F, Spanagel R*, Mann K, Ende G, Sommer WH (2012) Translational magnetic resonance spectroscopy reveals excessive central gluta- mate levels during alcohol withdrawal in humans and rats. Biol Psychiatry 71:1015-1021.
Nanguneri S, Flottmann B, Horstmann H, Heilemann M, Kuner T (2012) Three-dimensional, tomographic super-resolution fluorescence imaging of serially sectioned thick samples. PloS one 7:e38098.
Abraham NM, Egger V, Shimshek DR, Renden R, Fukunaga. I, Sprengel R*, Seeburg PH, Klug- mann M, Margrie TW, Schaefer AT, Kuner T (2010) Synaptic inhibition in the olfactory bulb accelerates odor discrimination in mice. Neuron 65:399-411.
Sommer WH, Rimondini R, Hansson AC*, Hipskind PA, Gehlert DR, Barr CS, Heilig M (2008) Upregulation of Voluntary Alcohol Intake, Behavioral Sensitivity to Stress, and Amygdala Crhr1 Expression Following a History of Dependence. Biol Psychiatry 63:139-145.
Kuner T, Li Y, Gee KR, Bonewald LF, Augustine GJ (2008) Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release. Proc Natl Acad Sci U S A 108:347-352.
Björk K ,Rimondini R, Hansson AC*, Terasmaa A, Hyytiä P, Heilig M, Sommer WH (2008) Modu- lation of voluntary ethanol consumption by beta-arrestin 2. FASEB J 22:2552-2560.
Dübel J, Haverkamp S, Schleich W, Augustine, GJ, Feng G, Kuner T , Euler T (2006) Two-pho- ton imaging reveals somato-dendritic chloride gradient in retinal ON-type bipolar cells ex- pressing the biosensor Clomeleon. Neuron 49:81-94.
Wimmer VC, Nevian T, Kuner T (2004) Targeted in vivo expression of proteins in the calyx of Held. Pflugers Arch 449:319-333.
Kuner T & Augustine GJ (2000) A genetically encoded ratiometric indicator for chloride: capturing chloride transients in hippocampal neurons. Neuron 27:447-459.
* Principal investigators of other projects within the CRC