Characterization and modulation of neuronal ensembles involved in reward learning

click for full picture
Rewards which can be conditioned to specific cues drive our daily life activities.

We hypothesize that the underlying memorization process results in the formation of specific cue-reward encoding neuronal ensembles within the mesocorticolimbic reward system.

We will use novel tools to study such neuronal ensembles. Activated groups of neurons will be identified by means of a new c-fos-GFP rat line. Labelled neuronal ensembles will be further characterized with respect to molecular markers and electrophysiological characteristics, and their role will be verified by selective Daun02 inactivation.
Halbout B, Bernardi R, Hansson AC, Spanagel R (2014) Incubation of cocaine-seeking following brief cocaine experience in mice is enhanced by mGluR1 blockade. J Neurosci 34:1781- 1790.

Cannella N, Halbout B, Uhrig S, Evrard L, Corsi M, Corti C, Deroche-Gamonet V, Hansson AC, Spanagel R (2013) The mGluR2/3 agonist LY379268 induced anti-reinstatement effects in rats exhibiting addiction-like behavior. Neuropsychopharmacology 38:2048-2056.

Meinhardt MW, Hansson AC, Perreau-Lenz S, Bauder-Wenz C, Stählin O, Heilig M, Harper C, Drescher KU, Spanagel R, Sommer WH* (2013) Rescue of infralimbic mGluR2 deficit restores control over drug-seeking behavior in alcohol dependence. J Neurosci 33:2794- 2806.

Herwerth M, Jensen V, Novak M, Konopka W, Hvalby Ø, Köhr G (2012) D4 dopamine receptors modulate NR2B NMDA receptors and LTP in stratum oriens of hippocampal CA1. Cereb Cortex 22:1786-1798.

Mameli M, Halbout B, Creton C, Spanagel R, Lüscher C (2009) Cocaine-evoked synaptic plas- ticity: persistence in the VTA triggers adaptations in the nucleus accumbens. Nat Neurosci 12:1036-41.

Bilbao A, Rodriguez Parkitna J. Engblom D, Sanchis-Segura C, Perrau-Lenz S, Schneider M, Konopka W, Westphal M, Breen G, Desrivieres S, Klugmann M, Bading H*, Rodriguez de Fonseca F, Vallada H, Schumann G, Schütz G, Spanagel R (2008) Loss of the Ca2+/ calmodulin-dependent protein kinase type IV in dopaminoceptive neurons enhances be- havioral effects of cocaine. Proc Natl Acad Sci U S A 105:17549-17554.

Engblom D, Sanchis-Segura C, Bilbao-Leis A, Dahan L, Perreau-Lenz S, Balland B, Mameli M, Rodriguez Parkitna J, Parlato R, Sprengel R*, Lüscher C, Schütz G, Spanagel R (2008) Glutamate receptors on dopaminergic neurons control the persistence of drug-seeking. Neuron 59:497-508.

Hansson AC, Cippitelli A, Sommer WH*, Fedeli A, Björk K, Soverchia L, Terasmaa A, Massi M, Heilig M, Ciccocioppo R (2006) Variation at the rat Crhr1 locus and sensitivity to relapse into alcohol seeking induced by environmental stress. Proc Natl Acad Sci U S A 103, 15236-15241.

Pawlak V, Jensen V, Schupp BJ, Kvello A, Hvalby Ø, Seeburg PH, Köhr G (2005) Frequency dependent impairment of hippocampal LTP from NMDA receptors with reduced calcium permeability. Eur J Neurosci 22:476-484.

Pawlak V, Schupp BJ, Single FN, Seeburg PH, Köhr G (2005) Impaired synaptic scaling in mouse hippocampal neurons expressing NMDA receptors with reduced calcium permeability. J Physiol 562:771-783.

Hansson AC, Sommer W*, Rimondini R, Andbjer B, Strömberg I, Fuxe K (2003) c-fos reduces corticosterone-mediated effects on neurotrophic factor expression in the rat hippocampal CA1 region. J Neurosci 23:6013-6022.

Sommer W*, Rimondini R, O’Connor W, Hansson AC, Ungerstedt U, Fuxe K (1996) Intrastriatal- ly injected c-fos antisense oligonucleotide interferes with striatonigral but not striatopallidal gamma-aminobutyric acid transmission in the conscious rat. Proc Natl Acad Sci U S A 93:14134-14139.

*Principal investigators of other projects within the CRC